What are the developments and the results on the Young Talent Program?


In WP2 the talent program award was used to extend and enrich the datasets in the two ways: (1) we have completed the additional proteomics profile in Lifelines DEEP samples, using cardiovascular circulating proteins from the OLINK platform. We identified substantial effect of genetics and microbiome on the levels of circulating proteins, the data analysis is in progress. Secondly, we used this money to extend the Lifelines microbiome collection for additional 10,000 samples. The technician was appointed to help collecting samples and isolate microbial DNA. This project is currently in progress. Furthermore, with help from the YT program A. Zhernakova received both ERC (2016) and VIDI (2017) grants for the microbiome projects.


In the last years the young talent grant of Prof. Nieuwdorp has been used to further investigate the role of gut microbiota on epigenetic markers and the relation with cardio-metabolism. the previous postdoc on the project (Dr. Siroon Bekkering together with Prof. Nieuwdorp and Prof. Stroes) has several papers in progress that also study the role of epigenetic regulation of monocytes in cardio-metabolism. Currently, we have a paper on lean donor FMT and insulin sensitivity in press in Cell Metabolism 2017 (Kootte/Nieuwdorp et al). In this project both gut microbiota composition as well as plasma metabolites were available, and for the CVON young talent program he is now investigating the relation between epigenetic (methylation profiles in PBMCs) signature before and after lean donor FMT in relation to gut microbiota and plasma metabolite concentrations. This is done together with Dr. Andrei Prodan (current postdoc on the project).


Based on the finding that plasma levels of lysosomal enzymes can be used as marker for metabolic inflammation, Ronit Shiri-Sverdlov obtained a grant, supported by the TKI (amount: € 1.000.000). Future research will focus on the implementation of the lysosomal markers as diagnostic tool in the clinic. To obtain this goal, multiple national and international cohorts will be used. In addition, Yvonne Oligschläger (post-doc in this group) received a CVON RECONNECT grant.